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EL and YC wrote the manuscript. IT and CV performed the clinical part of the study. SV performed the histopathological part of the study. BL and MF read and improved the manuscript. YC supervised all the steps of the study. All authors read and approved the final manuscript. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Electronic supplementary material. Adriana Nabuco, Email: moc.

Sophie Vandenabeele, Email: eb. Bertrand Losson, Email: eb.


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Shukri Sharif, Email: rf. Michel Franc, Email: rf. Caroline Vanvinckenroye, Email: eb. Yannick Caron, Email: gro. National Center for Biotechnology Information , U. Journal List Parasit Vectors v. Parasit Vectors. Published online Jul Author information Article notes Copyright and License information Disclaimer. Corresponding author. Received Feb 7; Accepted Jul 4. This article has been cited by other articles in PMC. Variable sites are highlighted in yellow.

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DOCX kb. Additional file 2: Alignment of ITS2. Additional file 3: Alignment of 5. DOCX 66 kb. Abstract Background Besnoitiosis is caused by different species of intracellular protozoan parasites belonging to the family Sarcocystidae and affecting multiple host species worldwide. Case presentation A two-year-old male donkey was purchased in May in poor body condition cachexia, alopetic areas and pruritus mainly on neck and head by the present owner in Le Roeulx Belgium from a milk producing donkey farm in Frasnes-lez-Buissenal Belgium.

Conclusion This is the first evidence of Besnoitia bennetti infection Protozoa: Apicomplexa in donkeys Equus asinus in Belgium. Electronic supplementary material The online version of this article Background Besnoitiosis is caused by different species of intracellular protozoan parasites belonging to the family Sarcocystidae phylum Apicomplexa affecting multiple host species worldwide.

Open in a separate window. Clinical descriptions Shortly after its purchase, the animal was shorn revealing crusts and hyperkeratosis on both flanks and the neck. Laboratory examination, diagnosis and prognosis Skin scraping Skin scrapings were performed in different places mainly flanks and neck where the lesions were the most obvious on both animals.

Histopathology Several skin biopsies were taken using an 8 mm biopsy punch. Blood sampling and analysis Several blood samples were taken for haematology and biochemistry analysis from both patients. Discussion The current gold standard for diagnosing besnoitiosis in donkeys is histological identification of Besnoitia cysts within the dermis of individuals with lesions, generally achieved via skin biopsy [ 15 , 23 — 25 ]. Conclusions Difficulties in confirming diagnoses and the absence of efficient treatment options are probably responsible for the underreporting of besnoitiosis in European donkeys.


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Additional file 3: 67K, docx Alignment of 5. Acknowledgments We would like to thank Colette de Vincke, the owner of the two donkeys, for her kindness and help in handling the patients, and Amelia Coggon for review and improvement of the English language. Notes Ethics approval and consent to participate Not applicable.

Consent for publication Not applicable.

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Competing interests The authors declare that they have no competing interests. Footnotes Electronic supplementary material The online version of this article References 1. EFSA J. A century of bovine besnoitiosis: an unknown disease re-emerging in Europe.

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Trends Parasitol. The role of wild ruminants as reservoirs of Besnoitia besnoiti infection in cattle.

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Vet Parasitol. Comparative evaluation of immunofluorescent antibody and new immunoblot tests for the specific detection of antibodies against Besnoitia besnoiti tachyzoites and bradyzoites in bovine sera. A review on bovine besnoitiosis: a disease with economic impact in herd health management, caused by Besnoitia besnoiti Franco and Borges Parasitology.


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Genetic characterization of the causative agent of besnoitiosis in goats in Iran on the basis of internal transcribed spacer rDNA and its comparison with Besnoitia species of other hosts. Parasitol Res. First confirmed case of bovine besnoitiosis in an imported bull in Belgium. Vlaams Diergeneeskd Tijdschr. Bennett SC. A peculiar equine Sarcosporidium in the Anglo-Egyptian Sudan.

Vet J. Globidosis - a cause of dermatitis in horses. Bigalke RD. Studies on equine besnoitiosis.

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J Parasitol. Besnoitiosis in a horse. J South Afr Vet Assoc. Diseases due to protozoa. For guidance on face coverings, visit the CDC. Wash your hands - Wash your hands often with soap and water for at least 20 seconds especially after you have been in a public place, or after blowing your nose, coughing, or sneezing. Clean and disinfect - This includes tables, doorknobs, light switches, countertops, handles, desks, phones, keyboards, toilets, faucets, and sinks.

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In the deambulatory, five other chapels have been expanded. The chapel of Saint Adrian contains the only Baroque altar to have survived the later decoration of the church. A brochure in the church gives the locations of thirty other works. A few years ago the inhabitants of Geraardsbergen devoted a book of photos to their church. Source: Severien Malfait. The Muur van Geraardsbergen is a steep, narrow road with cobblestones in Geraardsbergen, Belgium.

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It is also known as Kapelmuur, Muur-Kapelmuur or simply Muur. The hill starts near the river Dender at 18 m and reaches the top of the Oudenberg at m after 1, m at 9. This climb is often part of the Tour of Flanders professional cycling race. The Muur van Geraardsbergen has often been a climb in the Tour of Flanders. It has been used since , although not consistently until The "Muur" was the final climb in and , before the finish in Meerbeke.

An extra stretch to the chapel at the summit was added in and climb became known as the "Muur-Kapelmuur". It was a regular feature from until Source: Wikipedia.